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ppa12092-sup-0001-FigS1.pdfapplication/PDF12537KFigure S1. Two-dimensional plot representing the percentage sequence identities between the Brazilian CPMMV isolates and the most closely related carlaviruses. Nucleotide sequence identities are shown above the diagonal and amino acid sequence identities below. The six Brazilian isolates are indicated in red. (a) ORF1, RNA-dependent RNA polymerase (RdRp); (b, c, d) ORFs 2–4, triple gene block (TGB1, TGB2 and TGB3, respectively); (e) ORF5, coat protein (CP); (f) ORF6, nucleic acid-binding protein (NABP).
ppa12092-sup-0002-FigS2.pdfapplication/PDF3797KFigure S2. Phylogenetic relationships, based on the sequences of individual ORFs, of the Brazilian CPMMV isolates with different carlaviruses, determined using Bayesian inference (implemented in MrBayes v. 3.1, with selection of models GTR+I+G for all ORFs and 10 million generations). Indian citrus ringspot virus (ICRSV; genus Mandarivirus, family Alphaflexiviridae) was used as the out-group. Bayesian posterior probability values are given between nodes. The six Brazilian isolates are indicated in red. The accession numbers of the sequences are shown next to their acronym. (a) ORF1, RNA-dependent RNA polymerase (RdRp); (b, c, d) ORFs 2–4, triple gene block (TGB1, TGB2 and TGB3, respectively); (e) ORF5, coat protein (CP); (f) ORF6, nucleic acid-binding protein (NABP).
ppa12092-sup-0003-TableS1.docxWord document18KTable S1. Primers used in RT-PCR and predicted amplicon size for viral detection and cloning.
ppa12092-sup-0004-TableS2.docxWord document17KTable S2. Carlavirus sequences used for sequence comparisons, phylogenetic and recombination analyses.

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