ppl12019-sup-0001-FigureS1.docWord document16KFig. S1. Phylogenetic analysis of MfGolS in Medicago falcata with GolSs in Arabidopsis and other Medicago species. The bar represents the branch length equivalent to 0.05 amino acid changes per residue.
ppl12019-sup-0002-FigureS2.docWord document125KFig. S2. Characterization of MfGolS1. The Multiple alignments of amino acid sequence of MfGolS1 with five other plant GolSs. MsGolS, MtGolS1, AtGolS1, AtGolS2 and AtGolS3 indicated GolS from Medicago sativa (i.e. masGolS, AAM97493), Medicago truncacula (AES61481), Arabidopsis thaliana (AEC10811, AEE33413 and AEE28432), respectively. Identical amino acid residues among the six GolSs are shaded with black color, while those among the five GolSs are shaded with gray color. The putative serine phosphorylation site is shown by an arrow. A characteristic putative serine phosphorylation site (DGD) and a characteristic hydrophobic pentapeptide (APSAA) are shown as a bar.
ppl12019-sup-0003-FigureS3.docWord document49KFig. S3. Nucleotide and deduced amino acid sequence of the MfGolS1 gene.
ppl12019-sup-0004-FigureS4.docWord document69KFig. S4. Analysis of the transgenic tobacco (lines 22-2, 28-1 and 40-1) overexpressing MfGolS1 in comparison with the wild-type control (W). Five micrograms of genomic DNA from each plant line was digested with EcoRI for DNA blot hybridization (A). Twenty micrograms of total RNA was used for RNA blot hybridization (B).

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