Structure-function relationships during transgenic telomerase expression in Arabidopsis

Authors

  • Dagmar Zachová,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
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  • Miloslava Fojtová,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
    2. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
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  • Martina Dvořáčková,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
    2. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
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  • Iva Mozgová,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
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    • Present address: Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Box 7080, SE-75007 Uppsala, Sweden

  • Inna Lermontova,

    1. Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
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  • Vratislav Peška,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
    2. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
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  • Ingo Schubert,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
    2. Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
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  • Jiří Fajkus,

    1. Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
    2. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
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  • Eva Sýkorová

    Corresponding author
    1. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    • Faculty of Science and Central European Institute of Technology, Masaryk University, Brno, Czech Republic
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Correspondence

Corresponding author,

e-mail: evin@ibp.cz

Abstract

Although telomerase (EC 2.7.7.49) is important for genome stability and totipotency of plant cells, the principles of its regulation are not well understood. Therefore, we studied subcellular localization and function of the full-length and truncated variants of the catalytic subunit of Arabidopsis thaliana telomerase, AtTERT, in planta. Our results show that multiple sites in AtTERT may serve as nuclear localization signals, as all the studied individual domains of the AtTERT were targeted to the nucleus and/or the nucleolus. Although the introduced genomic or cDNA AtTERT transgenes display expression at transcript and protein levels, they are not able to fully complement the lack of telomerase functions in tert −/− mutants. The failure to reconstitute telomerase function in planta suggests a more complex telomerase regulation in plant cells than would be expected based on results of similar experiments in mammalian model systems.

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