Get access

Ectopic expression of wheat TaCIPK14, encoding a calcineurin B-like protein-interacting protein kinase, confers salinity and cold tolerance in tobacco

Authors

  • Xiaomin Deng,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
    • These authors equally contributed to this work.

  • Shiyi Zhou,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
    • These authors equally contributed to this work.

  • Wei Hu,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Jialu Feng,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Fan Zhang,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Lihong Chen,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Chao Huang,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Qingchen Luo,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Yanzhen He,

    1. The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Guangxiao Yang,

    Corresponding author
    • The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author
  • Guangyuan He

    Corresponding author
    • The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, China
    Search for more papers by this author

Correspondence

Corresponding authors,

e-mail: ygx@hust.edu.cn, hegy@hust.edu.cn

Abstract

Calcineurin B-like protein-interacting protein kinases (CIPKs) are components of Ca2+ signaling in responses to abiotic stresses. In this work, the full-length cDNA of a novel CIPK gene (TaCIPK14) was isolated from wheat and was found to have significant sequence similarity to OsCIPK14/15. Subcellular localization assay revealed the presence of TaCIPK14 throughout the cell. qRT-PCR analysis showed that TaCIPK14 was upregulated under cold conditions or when treated with salt, PEG or exogenous stresses related signaling molecules including ABA, ethylene and H2O2. Transgenic tobaccos overexpressing TaCIPK14 exhibited higher contents of chlorophyll and sugar, higher catalase activity, while decreased amounts of H2O2 and malondialdehyde, and lesser ion leakage under cold and salt stresses. In addition, overexpression also increased seed germination rate, root elongation and decreased Na+ content in the transgenic lines under salt stress. Higher expression of stress-related genes was observed in lines overexpressing TaCIPK14 compared to controls under stress conditions. In summary, these results suggested that TaCIPK14 is an abiotic stress-responsive gene in plants.

Ancillary