ppl12070-sup-0001-TableS1.docPDF document121KTable S1. Accession Number of sequences included in the phylogenetic trees. Accession numbers for all sequences used for the tree in Fig. 2. All sequences were obtained from NCBI databases, with the exception of TrLi818, obtained from TIGR (
ppl12070-sup-0001-TableS2.docPDF document52KTable S2. PSBS and LHCSR expression in Chlorophyte and mosses.
ppl12070-sup-0002-figureS1.docPDF document744KFig. S1. NPQ kinetics in Streptophyte algae. NPQ induction kinetics of selected Streptophyte algae: data from Fig. 3, here reported as mean ± sd. (A) Species from the basal groups of Streptophyta: Klebsormidium flaccidum (Kf, black squares), and Interfilum terricolum (It, red circles). (B, C) species from groups closer to plants: Chara corallina (Cc, black squares) and Coleochaete scutata (Cs, red circles) in (B) and Zygnema circumcarinatum (Zc, black squares), Cosmarium botrytis (Cb, red circles) and Mesotaenium caldariorum (Mc, green triangles) in (C). In panels A–C, Arabidopsis thaliana (At, blue diamonds) is also reported as comparison. (D) NPQ kinetics recorder for a few model organisms: the moss Physcomitrella patens (Pp, black circles), the Chlorophyta Chlamydomonas reinhardtii (Cr, red triangles) and the vascular plant Arabidopsis thaliana (At, blue squares). In all measurements actinic light was switched off after 8 min. Note the different y-axis in the different panels.
ppl12070-sup-0003-figureS2.docPDF document46KFig. S2. PSBS accumulation in the basal Streptophyta. Western blotting analysis for PSBS detection in Klebsormidium and Interfilum samples by overloading (2.5× compared to Fig. 4, 7.5 µg of Chls) protein extracts on gel. Physcomitrella protein extract (4 µg of Chls) is also present as positive control.
ppl12070-sup-0004-figureS3.docPDF document226KFig. S3. Effect of HL treatment in Coleochaete scutata and Zygnema circumcarinatum. NPQ induction kinetics of selected species with no NPQ enhancement upon HL irradiation (open circles) compared to CL growth (black squares). (A) Coleochaete and (B) Zygnema. The other species tested in this work are reported in Fig. 5 or HL irradiation instead caused strong photoinhibition (Cosmarium, Interfilum and Chara).

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