These authors equally contributed to this work.
Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing
Article first published online: 7 JUN 2013
© 2013 Scandinavian Plant Physiology Society
Volume 150, Issue 1, pages 119–132, January 2014
How to Cite
Hou, P.-Q., Lee, Y.-I., Hsu, K.-T., Lin, Y.-T., Wu, W.-Z., Lin, J.-Y., Nam, T. N. and Fu, S.-F. (2014), Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing. Physiologia Plantarum, 150: 119–132. doi: 10.1111/ppl.12071
- Issue published online: 16 DEC 2013
- Article first published online: 7 JUN 2013
- Accepted manuscript online: 17 MAY 2013 12:37PM EST
- Manuscript Accepted: 3 MAY 2013
- Manuscript Revised: 2 MAY 2013
- Manuscript Received: 26 JAN 2013
- Taiwan National Science Council. Grant Number: NSC 101-2321-B-018-002-MY2
DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.