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ppl12084-sup-0001-TableS1.docWord document428KTable S1. List of primers used and their properties. Primers were designed with Primer3 software. Primer specificity was checked by BLAST-ing against all apples ESTs and known cDNA sequences. All primers were optimized toward an annealing temperature of 60°C.
ppl12084-sup-0002-FigS1.docWord document428KFig. S1. Overview of the ethylene measurements (A), sampling for the intermediates and enzymes on individual replicates (B) and on pooled samples (C) during the first part of the experiment (the application of CA conditions).
ppl12084-sup-0003-FigS2.docWord document428KFig. S2. Overview of the ethylene measurements (A), sampling for the intermediates and enzymes on individual replicates (B) and on pooled samples (C) during the second part of the experiment (the removal of CA conditions).
ppl12084-sup-0004-FigS3.docWord document428KFig. S3. Overview of the changes in ethylene biosynthesis during CA storage of Jonagold, harvest at three different moments in time (early: September 5, 2008; optimal: September 19, 2008; late: October 3, 2008). Values at day 0 represent the measurements on the day of harvest for each group. On the short term application of the CA conditions caused the ACS activity of late harvested control apples to increase drastically (A). This led to an increased ACC concentration (B) as well as increased ethylene biosynthesis (D). ACO activity was not affected upon application of CA conditions but increased steadily throughout storage (C).

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