ppl12093-sup-0001-tS1.xlsExcel spreadsheet29KTable S1. Main primers for PCR used in this study.
ppl12093-sup-0002-tS2.xlsExcel spreadsheet37KTable S2. Transcripts of CaWRKY27 in pepper plants exposed to biotic and various abiotic stresses by qPCR experiments.
ppl12093-sup-0003-tS3.xlsExcel spreadsheet27KTable S3. Transcript levels of defense marker genes in CaWRKY27-OE-1 compared to wild-type tobacco plants by qPCR experiments.
ppl12093-sup-0004-tS4.xlsExcel spreadsheet30KTable S4. Transcripts of HR-associated genes and stress-related genes in wild-type and CaWRKY27-OE-1 tobacco plants at 24 or 48 h post-inoculation by qPCR experiments.
ppl12093-sup-0005-fS1.docWord document3941KFig. S1. Nucleotide and amino acid sequence of pepper CaWRKY27 (Q3SAJ9_CAPAN) cDNA clone. (A) Nucleic acids are shown in the top line and the deduced amino acids are presented below. The stop codon is indicated by the asterisk. The WRKY domain is shown in bold, and the WRKYGQK sequences are underlined. The cysteines and histidines of the zinc-finger-like motif are indicated by black triangles. The putative nuclear localization signal (NLS) site (PKRSR) is indicated by a green line.
ppl12093-sup-0006-fS2.docWord document3941KFig. S2. Subcellular localization of CaWRKY27. (A) Schematic representation of p35S::GFP and p35S::GFP-CaWRKY27 constructs. (B) GFP-CaWRKY27 exclusively localized in the nucleus of Nicotiana benthamiana leaves, whereas GFP alone localized throughout the whole cell; DAPI staining indicates nuclei. All images were captured using fluorescence microscopy 24 h after agroinfiltration. Bars = 20 µm.
ppl12093-sup-0007-fS3.docWord document3941KFig. S3. CaWRKY27 trans-activation experiments performed by particle bombardment with onion epidermal cells. (A) Schematic diagram of reporter and overexpression constructs used for co-transfection in particle-bombarded onion epidermal cells. (B) Onion epidermal cells co-transformed with the indicated reporter and effector plasmids. Dark blue staining was only observed after co-transfection of 2×W-p35Score::GUS with p35S::CaWRKY27.
ppl12093-sup-0008-fS4.docWord document3941KFig. S4. Effects of Ralstonia solanacearum strain FJC100301 on two cultivars of pepper. Ralstonia solanacearum strain FJC100301 was taken from stem exudates (STEM) or the vascular portion (VP) of wilted pepper plants. (A, B) In May 2010, 8-week-old pepper cultivars of 76a (A) and Gui-1-3 (B) were infected with R. solanacearum through root inoculation in Quanzhou, Fujian, China. The photographs were taken 11 days after infection. (C, D) The fifth leaves of 7-week-old pepper cultivars of 76a (C) and Gui-1-3 (D) inoculated with R. solanacearum. The photographs were taken 4 days after inoculation.

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