ppl12213-sup-0001-AppendixS1.docxWord 2007 document14KAppendix S1. List of primers used in this investigation.
ppl12213-sup-0002-FigureS1.pptPowerPoint presentation264KFigure S1. PCR analysis indicating the transfer of transgene cassette into the tobacco plants. Lane M: λ DNA EcoRI/ HindIII digest, Lane W: Amplification of untransformed plant DNA, Lane V: Amplification of vector transformed plant DNA, Lanes HMB2, HMC1 and HMD1 denotes amplified DNA from WsHMGR2 transformed plant lines, Lanes DXC1, DXD1 and DXD5 have amplified DNA from WsDXR2 transformed plant lines. The cassette was amplified with primers from CaMV35S promoter and nos terminator. Hence, untransformed plant DNA does not show amplification, vector transformed plant DNA shows 2486 bp amplicon where as WsHMGR2 transformed plant lines show 2223 bp amplicons and WsDXR2 shows 2100 bp amplicons.
ppl12213-sup-0003-FigureS2.pptPowerPoint presentation150KFigure S2. GC chromatograms of (A) cholesterol (Ch); (B) tocopherol (To) and (C) cholesterol and tocopherol mixture injected together.
ppl12213-sup-0004-FigureS3.pptPowerPoint presentation443KFigure S3. Representative GC chromatograms showing (A) standards for estrone (ES), cholesterol (Ch), campesterol (Ca), stigmasterol (St), sitosterol (Si), cycloartenol (cy); (B) sterols from vector transformed plant; (C) plant transformed with WsHMGR2; (D) plant transformed with WsDXR2. The right-hand panel shows GC-MS profiles of different sterols analyzed in this investigation.
ppl12213-sup-0005-FigureS4.docxWord 2007 document21KFigure S4. Multiple sequence alignment of HMGRs showing RNAi fragment and the primer position for semiquantitative PCR analysis.
ppl12213-sup-0006-FigureS5.docxWord 2007 document18KFigure S5. Multiple sequence alignment of DXRs showing RNAi fragment and the primer position for semiquantitative PCR analysis.

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