Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells
Article first published online: 24 DEC 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Special Issue: Canine and Feline Reproduction VII: Reproductive Biology and Medicine of Domestic and Exotic Carnivores. Proceedings of the 7th Quadrennial International Symposium on Canine and Feline Reproduction. Whistler, Canada. 26-29 July 2012.
Volume 47, Issue Supplement s6, pages 84–87, December 2012
How to Cite
Gonçalves, N., Bressan, F., Souza, A., Martins, D., Miglino, M., Meirelles, F., Perecin, F. and Ambrósio, C. (2012), Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells. Reproduction in Domestic Animals, 47: 84–87. doi: 10.1111/rda.12002
- Issue published online: 24 DEC 2012
- Article first published online: 24 DEC 2012
- State of São Paulo Research Foundation. Grant Number: 2012/01060-4
- FAPESP graduate fellowships. Grant Numbers: 2011/22915-5, 2009/11631-6
The aim of this study was to further clarify the mechanisms involved in inducing pluripotency using canine foetal fibroblast cells. The two pluripotency-related transcription factors, OCT4 and SOX2, coupled to a fluorescent reporter gene were transduced, individually or in combination, using a lentiviral system. Stable transgenic cell lineages were obtained and canine cells showed to be highly responsive to the integration and expression of human SOX2 and OCT4, also depending on the amount of virus used for incubation. Such positive results are essential for the establishment of pluripotency induction through the incorporation of known transcription factors into the genome of somatic cells.