Scriptaid and 5-aza-2′deoxycytidine Enhanced Expression of Pluripotent Genes and in vitro Developmental Competence in Interspecies Black-Footed Cat Cloned Embryos
Article first published online: 24 DEC 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Special Issue: Canine and Feline Reproduction VII: Reproductive Biology and Medicine of Domestic and Exotic Carnivores. Proceedings of the 7th Quadrennial International Symposium on Canine and Feline Reproduction. Whistler, Canada. 26-29 July 2012.
Volume 47, Issue Supplement s6, pages 130–135, December 2012
How to Cite
Gómez, M., Biancardi, M., Jenkins, J., Dumas, C., Galiguis, J., Wang, G. and Earle Pope, C. (2012), Scriptaid and 5-aza-2′deoxycytidine Enhanced Expression of Pluripotent Genes and in vitro Developmental Competence in Interspecies Black-Footed Cat Cloned Embryos. Reproduction in Domestic Animals, 47: 130–135. doi: 10.1111/rda.12027
- Issue published online: 24 DEC 2012
- Article first published online: 24 DEC 2012
Somatic cell nuclear transfer offers the possibility of preserving endangered species including the black-footed cat, which is threatened with extinction. The effectiveness and efficiency of somatic cell nuclear transfer (SCNT) depends on a variety of factors, but ‘inappropriate epigenetic reprogramming of the transplanted nucleus is the primary cause of the developmental failure of cloned embryos. Abnormal epigenetic events such as DNA methylation and histone modifications during SCNT perturb the expression of imprinted and pluripotent-related genes that, consequently, may result in foetal and neonatal abnormalities. We have demonstrated that pregnancies can be established after transfer of black-footed cat cloned embryos into domestic cat recipients, but none of the implanted embryos developed to term and the foetal failure has been associated to aberrant reprogramming in cloned embryos. There is growing evidence that modifying the epigenetic pattern of the chromatin template of both donor cells and reconstructed embryos with a combination of inhibitors of histone deacetylases and DNA methyltransferases results in enhanced gene reactivation and improved in vitro and in vivo developmental competence. Epigenetic modifications of the chromatin template of black-footed cat donor cells and reconstructed embryos with epigenetic-modifying compounds enhanced in vitro development, and regulated the expression of pluripotent genes, but these epigenetic modifications did not improve in vivo developmental competence.