Use of Fluorescent Stainings and Flow Cytometry for Canine Semen Assessment
Article first published online: 24 DEC 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Special Issue: Canine and Feline Reproduction VII: Reproductive Biology and Medicine of Domestic and Exotic Carnivores. Proceedings of the 7th Quadrennial International Symposium on Canine and Feline Reproduction. Whistler, Canada. 26-29 July 2012.
Volume 47, Issue Supplement s6, pages 215–221, December 2012
How to Cite
Niżański, W., Partyka, A. and Rijsselaere, T. (2012), Use of Fluorescent Stainings and Flow Cytometry for Canine Semen Assessment. Reproduction in Domestic Animals, 47: 215–221. doi: 10.1111/rda.12048
- Issue published online: 24 DEC 2012
- Article first published online: 24 DEC 2012
- Manuscript Accepted: 27 JUN 2012
- Manuscript Received: 29 MAY 2012
- Polish Government
- Ministry of Science and Higher Education in Poland. Grant Number: N N308 576540
Over the last few decades, appreciable progress has been noted in canine semen assessment techniques. The common use of accurate and sensitive diagnostic methods, such as computer-assisted sperm analysis (CASA), flow cytometry and sperm penetration tests have become routine procedures in specialized andrology laboratories. Many fluorescent probes have been applied to the assessment of specific sperm characteristics in dogs. Flow cytometry enables the observation of cell characteristics such as size, shape and function of the spermatozoon, that can be revealed by a fluorochrome or fluorescent label. The analysis of events detected on dot plots gives accurate and highly reliable information on membrane integrity, acrosomal status, mitochondrial activity, capacitation status, lipid peroxidation, apoptosis and DNA damage. Despite the development of these modern and accurate tools, it is still questionable if the ideal method of semen evaluation, allowing predicting of the fertilizing potential of semen, has been established.