Laparoscopic Oviductal Embryo Transfer and Artificial Insemination in Felids – Challenges, Strategies and Successes
Article first published online: 24 DEC 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Special Issue: Canine and Feline Reproduction VII: Reproductive Biology and Medicine of Domestic and Exotic Carnivores. Proceedings of the 7th Quadrennial International Symposium on Canine and Feline Reproduction. Whistler, Canada. 26-29 July 2012.
Volume 47, Issue Supplement s6, pages 136–140, December 2012
How to Cite
Swanson, W. (2012), Laparoscopic Oviductal Embryo Transfer and Artificial Insemination in Felids – Challenges, Strategies and Successes. Reproduction in Domestic Animals, 47: 136–140. doi: 10.1111/rda.12069
- Issue published online: 24 DEC 2012
- Article first published online: 24 DEC 2012
- Manuscript Accepted: 10 JUL 2012
- Manuscript Received: 6 JUN 2012
- Institute of Museum and Library Sciences
- National Institutes of Health
Embryo transfer (ET) and artificial insemination (AI) are potentially invaluable techniques for the propagation and management of genetically valuable domestic cat and endangered nondomestic cat populations. Many of the challenges that impair the effective application of ET and AI in felids may be overcome by using laparoscopic oviductal (LO) approaches. LO-ET and LO-AI are minimally-invasive procedures, requiring only two small skin incisions for insertion of a laparoscope and grasping forceps into the abdominal cavity to permit visualization and catheterization of the oviduct for embryo or semen deposition. With concurrent improvements in embryo culture systems and ovarian synchronization protocols, LO-ET has proven effective over the past decade for propagation of laboratory cats, cat models of hereditary disease and nondomestic cats. To date, viable offspring have been produced following LO-ET of non-frozen and frozen-thawed IVF-derived embryos in eight cat hereditary disease models and two nondomestic cat species, the ocelot and sand cat. LO-AI with low sperm numbers (c. 2–8 million motile) has shown similar efficacy to LO-ET, resulting in high pregnancy percentages (50–70%) following insemination of gonadotropin-treated domestic cats. Multiple kittens also have been produced in two hereditary disease models following LO-AI with frozen semen, and both ocelot and Pallas' cat kittens have been born after LO-AI with freshly-collected semen. The application of LO-ET and LO-AI to felids has resulted in substantial improvement in the efficiency of assisted reproduction for genetic management of these invaluable domestic cat and wild cat populations.