Effect of Different Manganese Concentrations during in vitro Maturation of Bovine Oocytes on DNA Integrity of Cumulus Cells and Subsequent Embryo Development

Authors

  • JP Anchordoquy,

    1. Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET, La Plata, Argentina
    2. Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina
    Search for more papers by this author
  • JM Anchordoquy,

    1. Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET, La Plata, Argentina
    2. Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina
    Search for more papers by this author
  • MA Sirini,

    1. Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET, La Plata, Argentina
    2. Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina
    Search for more papers by this author
  • G Mattioli,

    1. Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina
    Search for more papers by this author
  • SJ Picco,

    1. Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET, La Plata, Argentina
    2. Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina
    Search for more papers by this author
  • CC Furnus

    Corresponding author
    1. Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET, La Plata, Argentina
    2. Cátedra de Citología, Histología y Embriología “A”, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina
    • Author's address (for correspondence): CC Furnus, Instituto de Genética Veterinaria Prof. Fernando N. Dulout (IGEVET), Facultad de Ciencias Veterinarias, CONICET; Cátedra de Citología, Histología y Embriología “A”, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina. E-mail: cfurnus@fcv.unlp.edu.ar

    Search for more papers by this author

Contents

Manganese (Mn) is a trace element present in forages and cereals, and its concentration depends on soil status. Manganese deficiency in cattle, goats and ewes not only impairs oestrous cycle but reduces calf birth weight. The achievement of the first oestrus is delayed, and more attempts are necessary to obtain a successful conception. This study was conducted to investigate the effect of the availability of supplemental Mn during IVM on DNA damage of cumulus cells and total glutathione (GSH) content in oocytes and cumulus cells. The effect of supplementary Mn during IVM on subsequent embryo development was also studied. The results reported here indicate (i) DNA damage in cumulus cells decreased with 0, 2, 5 and 6 ng/ml Mn supplementation during IVM (p < 0.05). (ii) Intracellular GSH-GSSG content increased (p < 0.01) with different Mn concentrations in oocytes and cumulus cells. Also, cumulus cell number per cumulus oocyte-complexes (COC) did not differ either before or after IVM. (iii) Addition of Mn to maturation medium resulted in similar cleavage rates (p > 0.05) at 0, 2, 5 and 6 ng/ml Mn. However, subsequent embryo development to blastocyst stage was significantly higher (p < 0.01) in oocytes matured with 5 and 6 ng/ml Mn. (iv) There was also an increase (p < 0.05) in mean cell number per blastocyst obtained from oocytes matured with 5 and 6 ng/ml respect to zero Mn (IVM alone) and 2 ng/ml Mn. This study provides evidence that optimal embryo development to the blastocyst stage was partially dependent on the presence of Mn during IVM. Moreover, the availability of Mn during oocyte maturation ensures ‘normal’ intracellular GSH content in COCs and protects DNA integrity of cumulus cells.

Ancillary