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rda12244-sup-0001-FigureS1.docxWord document176KFigure S1 Semen collection was carried out twice per week during the control period and after the first and second hyperthermia. Individual dogs were castrated either 9 weeks after first hyperthermia (n = 2), 10 days after second hyperthermia (n = 3) or 40 days after the second hyperthermia (n = 2). (a) A general, but moderate, increase in the level of morphological abnormal sperm can be observed from day 11 onwards after the first hyperthermia (ejaculate no. 13–26). (b) Similarly, primary morphological sperm defects tended to stabilize at a relatively higher level from day 11 onwards after the first hyperthermia (ejaculate no. 13–26). This trend was not present in sperm with abnormal head shape. (c) Secondary morphological sperm defects were mainly represented by sperm with cytoplasmic droplets. All values are given as mean and standard deviation. Values that differ from the mean of the control period are indicated by an asterisk (p < 0.05). Values that tend to differ from the mean of the control period are indicated by a hash key (0.05 < p < 0.10).
rda12244-sup-0002-FigureS2.docxWord document47KFigure S2 Semen collection was carried out twice per week during the control period and after the first and second hyperthermia. For each ejaculate the percent of sperm with high DNA-fragmentation index (%DFI) was assessed. All values are given as mean and standard deviation. Values that are different from the mean of the control period are indicated by an asterisk (p < 0.05). Values that tend to differ from the mean of the control period are indicated by a hash key (0.05 < p < 0.10).
rda12244-sup-0003-TableS1.docWord document39KTable S1 Differences in histological findings in HE-stained sections of epididymal tissue from Beagle-dogs before and after a mild scrotal hyperthermia for 48 h. A total of 25 tubules were evaluated for each section (cranial, middle, caudal) of both epididymides from each dog.

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