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In Reproduction in Domestic Animals 48 Supplement 1 (Proceedings of the 17th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), Bologna, Italy 12 – 14th September 2013), the following error was published on page 64.

The text for the abstract of Tapia et al. (2013) was truncated as follows:

OC 1.3

The autophagy pathway regulates cell survival and motility in stallion spermatozoa

J Tapia 1 , A Miro-Moran 1 , C Balao da Silva 2 , C Ortega 2 , G Salido 1 , F Peña 2 , I Aparicio 1

1University of Extremadura, Caceres, Spain; 2Hospital Clinico Veterinario, Caceres, Spain

Autophagy is a well-conserved catabolic process involving the degradation and recycling of cytoplasmic proteins and organelles which plays important roles in somatic cells. However, nothing has been described so far in mammalian sperm cells. Our aim was to investigate whether autophagy takes place in equine spermatozoa and its putative role in these cells. Autophagy was assessed by Western blotting and immunofluorescence, using a specific antibody that recognizes LC3 I and II (the ratio between both proteins gives the flux of autophagy). Motility and viability were assessed by CASA and flow cytometry, respectively, in a BWW medium in presence or absence of autophagy activators (STF/Rapamycin) or inhibitors (3-MA/Chloroquine). Our results show that LC3 I and II are present in equine spermatozoa, mainly expressed in the flagellum in mature ejaculated sperm. In immature and morphologically abnormal cells, LC3 is highly expressed in the cytoplasmic.

This text was incorrect and should have read:

OC 1.3

The autophagy pathway regulates cell survival and motility in stallion spermatozoa

J Tapia 1 , A Miro-Moran 1 , C Balao da Silva 2 , C Ortega 2 , G Salido 1 , F Peña 2 , I Aparicio 1

1University of Extremadura, Caceres, Spain; 2Hospital Clinico Veterinario, Caceres, Spain

Autophagy is a well-conserved catabolic process involving the degradation and recycling of cytoplasmic proteins and organelles which plays important roles in somatic cells. However, nothing has been described so far in mammalian sperm cells. Our aim was to investigate whether autophagy takes place in equine spermatozoa and its putative role in these cells. Autophagy was assessed by Western blotting and immunofluorescence, using a specific antibody that recognizes LC3 I and II (the ratio between both proteins gives the flux of autophagy). Motility and viability were assessed by CASA and flow cytometry, respectively, in a BWW medium in the presence or absence of autophagy activators (STF/Rapamycin) or inhibitors (3-MA/Chloroquine). Our results show that LC3 I and II are present in equine spermatozoa, mainly expressed in the flagellum in mature ejaculated sperm. In immature and morphologically abnormal cells, LC3 is highly expressed in the cytoplasmic droplets. Incubation of stallion sperm in different conditions increased autophagy turnover, indicating that this is an active process. Inhibition of autophagy increased motility, whereas activation of autophagy decreased viability and motility. In conclusion, the autophagy pathway is present in equine spermatozoa and is actively involved, at least partially, in the regulation of cell viability and motility. Supported by Junta de Extremadura-FEDER (GR10010 and PCE1002) and by MICINN-FEDER (BFU2011-30261 and AGL 2010-20758).

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Tapia J, Miro-Moran A, Balao da Silva C, Ortega C, Salido G, Peña F, Aparicio I, 2013: The autophagy pathway regulates cell survival and motility in stallion spermatozoa. Reprod Domest Anim 48(Suppl. 1), 64.