These authors contributed equally to this work.
The Influence of Ovarian Stromal/Theca Cells During In Vitro Culture on Steroidogenesis, Proliferation and Apoptosis of Granulosa Cells Derived from the Goat Ovary
Version of Record online: 21 NOV 2013
© 2013 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Volume 49, Issue 1, pages 170–176, February 2014
How to Cite
Qiu, M., Liu, J., Han, C., Wu, B., Yang, Z., Su, F., Quan, F. and Zhang, Y. (2014), The Influence of Ovarian Stromal/Theca Cells During In Vitro Culture on Steroidogenesis, Proliferation and Apoptosis of Granulosa Cells Derived from the Goat Ovary. Reproduction in Domestic Animals, 49: 170–176. doi: 10.1111/rda.12256
- Issue online: 16 JAN 2014
- Version of Record online: 21 NOV 2013
- Manuscript Accepted: 13 OCT 2013
- Manuscript Received: 4 MAY 2013
- Department of National Science and Technology. Grant Number: 2011AA100303
Early follicular development is closely related to oocyte-granulosa cells-ovarian stromal cells/theca cells. The aim of the present study was to investigate the effects of ovarian cortical, medullary stromal and theca cells on oestradiol and progesterone biosynthesis, proliferation and apoptosis of goat ovary granulosa cells in vitro. Using Transwell coculture system, we evaluated steroidogenesis, cell proliferation and apoptosis, and some molecular expressions regarding steroidogenic enzyme, luteinizing hormone receptor and apoptosis-related genes in granulosa cells. The results indicated that ovarian stromal/theca cells were able to stimulate oestradiol and progesterone production, promote cell proliferation and inhibit apoptosis of granulosa cells. Among all the three kinds of cells, theca cells affected strongly on granulosa cell function, and ovarian medullary stromal cells had the weakest effect on granulosa cells. These findings would provide an important knowledge of cell interaction among follicular cells during follicular development.