The Influence of Ovarian Stromal/Theca Cells During In Vitro Culture on Steroidogenesis, Proliferation and Apoptosis of Granulosa Cells Derived from the Goat Ovary

Authors

  • M Qiu,

    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
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    • These authors contributed equally to this work.
  • J Liu,

    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
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    • These authors contributed equally to this work.
  • C Han,

    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
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  • B Wu,

    1. Arizona Center for Reproductive Endocrinology and Infertility, Tucson, AZ, USA
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  • Z Yang,

    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
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  • F Su,

    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
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  • F Quan,

    Corresponding author
    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
    • Author's address (for correspondence): Fusheng Quan, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China.

      E-mail: quanfusheng@nwsuaf.edu.cn and

      Yong Zhang, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.

      E-mail: zhangyong1956@nwsuaf.edu.cn

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  • Y Zhang

    Corresponding author
    1. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, China
    2. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China
    • Author's address (for correspondence): Fusheng Quan, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China.

      E-mail: quanfusheng@nwsuaf.edu.cn and

      Yong Zhang, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.

      E-mail: zhangyong1956@nwsuaf.edu.cn

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Contents

Early follicular development is closely related to oocyte-granulosa cells-ovarian stromal cells/theca cells. The aim of the present study was to investigate the effects of ovarian cortical, medullary stromal and theca cells on oestradiol and progesterone biosynthesis, proliferation and apoptosis of goat ovary granulosa cells in vitro. Using Transwell coculture system, we evaluated steroidogenesis, cell proliferation and apoptosis, and some molecular expressions regarding steroidogenic enzyme, luteinizing hormone receptor and apoptosis-related genes in granulosa cells. The results indicated that ovarian stromal/theca cells were able to stimulate oestradiol and progesterone production, promote cell proliferation and inhibit apoptosis of granulosa cells. Among all the three kinds of cells, theca cells affected strongly on granulosa cell function, and ovarian medullary stromal cells had the weakest effect on granulosa cells. These findings would provide an important knowledge of cell interaction among follicular cells during follicular development.

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