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Colloid Centrifugation Selects Normal Spermatozoa from Polymorphic Bull Ejaculates: A Case Study

Authors

  • JM Morrell,

    Corresponding author
    1. Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
    • Author's address (for correspondence): JM Morrell, Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, SE-75007 Uppsala, Sweden. E-mail: jane.morrell@slu.se

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  • H Rodriguez-Martinez,

    1. Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
    2. Department of Clinical and Experimental Medicine (IKE), Linköping University, Linköping, Sweden
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  • M Andersson

    1. Department of Production Animal Medicine, University of Helsinki, Saarentaus, Finland
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Contents

Semen from a Western Finncattle bull exhibiting a highly polymorphic spermiogram was processed by colloid centrifugation using Androcoll-B, a species-specific silane-coated silica colloid. In the first experiment, Single Layer Centrifugation (SLC) was used to identify which density colloids were needed to separate different cell populations. Colloids of the two chosen densities were then used in a density gradient resulting in two sperm subpopulations, one containing nearly all normally sized spermatozoa and the other enriched for the macrocephalic spermatozoa. Microcephalic spermatozoa did not appear in either of the selected subpopulations. Using a combination of SLC and DGC with this species-specific colloid, it was possible to separate the spermatozoa into different subpopulations, that is, a subpopulation containing nearly all normally sized spermatozoa, and another one enriched for the macrocephalic spermatozoa. Thus, colloid centrifugation could be used to select sufficient normal spermatozoa from a highly polymorphic ejaculate for AI, if desired.

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