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Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality

Authors

  • C Tomás,

    1. Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Segorbe, Castellón, Spain
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  • J Gómez-Fernández,

    1. Centro de Pruebas de Porcino, Área de Investigación Ganadera, Subdirección de Investigación y Tecnología, Instituto Tecnológico Agrario, Consejería de Agricultura y Ganadería, Junta de Castilla y León, Hontalbilla, Segovia, Spain
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  • E Gómez-Izquierdo,

    1. Centro de Pruebas de Porcino, Área de Investigación Ganadera, Subdirección de Investigación y Tecnología, Instituto Tecnológico Agrario, Consejería de Agricultura y Ganadería, Junta de Castilla y León, Hontalbilla, Segovia, Spain
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  • E Mocé,

    1. Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Segorbe, Castellón, Spain
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  • E de Mercado

    Corresponding author
    1. Centro de Pruebas de Porcino, Área de Investigación Ganadera, Subdirección de Investigación y Tecnología, Instituto Tecnológico Agrario, Consejería de Agricultura y Ganadería, Junta de Castilla y León, Hontalbilla, Segovia, Spain
    • Author's address (for correspondence): E de Mercado, de la Peña, Centro de Pruebas de Porcino. Carretera Riaza-Toro, s/n, 40353-Hontalbilla, Segovia, Spain. E-mail: ita-merpened@itacyl.es

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Contents

The aim of the present study was to evaluate the effect that the addition of cholesterol-loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen-thawed boar sperm. Pooled semen (n = 5) from three boars was used for the experiments. The semen was cryopreserved with an egg-yolk-based extender, it was diluted after thawing in Beltsville thawing solution (BTS) supplemented with different concentrations of CLC (0, 12.5, 25, 50 or 100 mg/500 × 106 sperm), and these samples were incubated at 37°C for 150 min. The following parameters of sperm quality were evaluated 30 and 150 min after incubation: sperm with intact plasma membrane (SIPM; %), sperm with normal acrosomal ridge (NAR; %), total motile sperm (TMS; %), progressively motile sperm (PMS; %) and kinetic parameters. Both SIPM and NAR increased (p < 0.05) when the thawing extender was supplemented with 12.5, 25 and 50 mg CLC/500 × 106 sperm. Nevertheless, motility decreased (p < 0.05) when the concentration of CLC exceeded 12.5 mg CLC/500 × 106 sperm. In conclusion, our results suggest that the supplementation of thawing extenders with CLC improves sperm viability and reduces acrosome damage after freezing/thawing.

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