Skin Tolerance is Supported by the Spleen
Article first published online: 26 MAR 2013
© 2013 The Authors. Scandinavian Journal of Immunology © 2013 Blackwell Publishing Ltd
Scandinavian Journal of Immunology
Volume 77, Issue 4, pages 238–245, April 2013
How to Cite
Buettner, M., Bornemann, M. and Bode, U. (2013), Skin Tolerance is Supported by the Spleen. Scandinavian Journal of Immunology, 77: 238–245. doi: 10.1111/sji.12034
- Issue published online: 26 MAR 2013
- Article first published online: 26 MAR 2013
- Accepted manuscript online: 19 FEB 2013 08:53AM EST
- Manuscript Accepted: 7 FEB 2013
- Manuscript Received: 9 OCT 2012
- Deutsche Forschungsgemeinschaft. Grant Number: SFB621/A10
Figure S1 Flow cytometry staining of lymphocytes in LN and the spleen. mLN, pLN and the spleen were removed from mice, and flow cytometry was performed after OT or ST induction. Cell suspensions were prepared, and lymphocytes were identified in FSC and SSC. Cells were stained for CD3, CD4, CD19 and IgG. Furthermore, CD4, CD25 and FoxP3 positive cells were measured. CD4+ CD25+ cells were gated, and FoxP3+ cells were calculated. Isotype control and FoxP3 staining are shown in the dot plots.
Figure S2 Germinal centres are detectable in the spleen of skin tolerized mice. Immunofluorescent staining for B220 (B cells in green; A and D) and GL-7, used to identify germinal centres (red; B and E), was performed in the spleen of mice from peripherally or orally tolerized animals. The merging shows that in the spleen of peripherally tolerized mice, germinal centres are seen, whereas in orally tolerized mice, only marginal GL-7 expression is found.
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