Phenotypic Differences in Leucocyte Populations among Healthy Preterm and Full-Term Newborns

Authors

  • C. Quinello,

    Corresponding author
    1. Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
    2. Laboratory of Medical Investigation (LIM-36), Instituto da Criança, Hospital das Clínicas, São Paulo, Brazil
    • Correspondence to: C. Quinello, LIM-36, Department of Pediatrics, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, Av. Dr. Enéas Carvalho de Aguiar, 647 - Instituto da Criança – 5° andar, CEP: 05403-900, São Paulo,  SP,  Brazil.  E-mail: caquinello@hotmail.com

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  • A. L. Silveira-Lessa,

    1. Laboratory of Medical Investigation (LIM-36), Instituto da Criança, Hospital das Clínicas, São Paulo, Brazil
    2. Department of Parasitology, Instituto de Ciências Biomédicas da Universidade de São Paulo, São Paulo, Brazil
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  • M. E. J. R. Ceccon,

    1. Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
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  • M. A. Cianciarullo,

    1. Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
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  • M. Carneiro-Sampaio,

    1. Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
    2. Laboratory of Medical Investigation (LIM-36), Instituto da Criança, Hospital das Clínicas, São Paulo, Brazil
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  • P. Palmeira

    1. Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
    2. Laboratory of Medical Investigation (LIM-36), Instituto da Criança, Hospital das Clínicas, São Paulo, Brazil
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Abstract

The immune system of neonates has been considered functionally immature, and due to their high susceptibility to infections, the aim of this study was to analyse the phenotypic differences in leucocyte populations in healthy preterm and full-term newborns. We evaluated the absolute numbers and frequencies of dendritic cells (DCs) and DC subsets, monocytes and T and B lymphocytes and subsets in the cord blood of healthy moderate and very preterm (Group 1), late preterm (Group 2) and full-term (Group 3) newborns and in healthy adults, as controls, by flow cytometry. The analyses revealed statistically higher absolute cell numbers in neonates compared with adults due to the characteristic leucocytosis of neonates. We observed a lower frequency of CD80+ myeloid and plasmacytoid DCs in Group 1 and reduced expression of TLR-4 on myeloid DCs in all neonates compared with adults. TLR-2+ monocytes were reduced in Group 1 compared with Groups 2 and 3, and TLR-4+ monocytes were reduced in Groups 1 and 2 compared with Group 3. The frequencies and numbers of naïve CD4+ T and CD19+ B cells were higher in the three groups of neonates compared with adults, while CD4+ effector and effector memory T cells and CD19+ memory B cells were elevated in adults compared with neonates, as expected. Our study provides reference values for leucocytes in cord blood from term and preterm newborns, which may facilitate the identification of immunological deficiencies in protection against extracellular pathogens.

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