Stratum corneum evaluation methods: overview
Article first published online: 13 APR 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Skin Research and Technology
Volume 19, Issue 3, pages 213–219, August 2013
How to Cite
Myer, K. and Maibach, H. (2013), Stratum corneum evaluation methods: overview. Skin Research and Technology, 19: 213–219. doi: 10.1111/srt.12011
- Issue published online: 10 JUL 2013
- Article first published online: 13 APR 2013
- Manuscript Accepted: 9 NOV 2012
- stratum corneum;
- tape stripping;
- barrier integrity;
- water loss
The stratum corneum serves as a main barrier for the skin, minimizing water loss and regulating absorption of substances. Because of its surface location, it is readily available for analysis. Consequently, many techniques are amenable to investigating its content and function. Here, we review the methods employed to evaluate the stratum corneum and its function.
We reviewed Pubmed and Embase search results for ‘stratum corneum, ‘method,’ methods,’ ‘technique,’ and ‘evaluation’ and extracted pertinent articles that discussed ways to examine the stratum corneum and its constituents.
Traditional and novel methods vary by accuracy, ease of use, time requirements, cost, invasiveness, and equipment requirements.
The methods reviewed all contribute to our current picture of the stratum corneum. Tape stripping continues to be the most widely used, but variations in the use of the corneocytes obtained further contribute to the diversity in evaluation methods.