tan12172-sup-0001-FigureS1.pdfPDF document143KFigure S1. Example screenshot of Non-T Genomic Detection kit analysis on ABI3130 genetic analyser. The kit amplifies an ultraconserved sequence (UCS) at 96 bp and an area of DNA excised in mature T cells (Non-T, 206 bp). The sample purity can be calculated using the heights of the peaks or areas under the peaks.
tan12172-sup-0002-FigureS2.pdfPDF document198KFigure S2. Comparison of anti-CD5 and secondary antibody (1:100 dilution) staining in CD3+ cell population isolated from one volunteer blood sample. Left-hand plots (A, D, G) show forward scatter (FSC) vs side scatter (SSC) and gating. Middle plots (B, E, H) show SSC vs FL1 (anti-CD5-FITC or Goat antimouse IgG-FITC) plots on gated cells and right-hand plots (C, F, I) show SSC vs FL1 for all cells.
tan12172-sup-0003-FigureS3.pdfPDF document253KFigure S3. Comparison of CD5 and secondary antibody staining in three volunteers. Black bars indicate the percentage of total cells stained, spotted bars indicate percentage gated cells stained. Data shown are mean ± standard deviation (CD5 n = 2, secondary n = 3).
tan12172-sup-0004-FigureS4.pdfPDF document130KFigure S4. Effect of blood storage time and temperature on purity of isolated CD3+ cell populations. CD3+ isolations were carried out daily from blood samples from a single healthy volunteer stored at different temperatures. Graph shows the purity of populations isolated from samples stored at 4°C (open squares) or 22°C (closed diamonds) as assessed by flow cytometry.
tan12172-sup-0005-FigureS5.pdfPDF document132KFigure S5. Chimerism in whole blood vs isolated CD3+ cell subset. Percentage donor chimerism in isolated CD3+ cell population (black squares) and whole peripheral blood (open diamonds) in one patient over time following allogeneic haematopoietic stem cell transplantation for acute myeloid leukaemia (AML) (days 48, 79, 100 and 156 post-transplant).

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