Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development

Authors

  • Isidora Romani,

    1. Dipartimento di Bioscienze, Università degli studi di Milano, I-20133 Milano, Italy
    Search for more papers by this author
    • These authors contributed equally to this work.

    • Present Address: Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, D-82152 Planegg-Martinsried, Germany.

  • Luca Tadini,

    1. Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, D-82152 Planegg-Martinsried, Germany
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Fabio Rossi,

    1. Dipartimento di Bioscienze, Università degli studi di Milano, I-20133 Milano, Italy
    Search for more papers by this author
  • Simona Masiero,

    1. Dipartimento di Bioscienze, Università degli studi di Milano, I-20133 Milano, Italy
    Search for more papers by this author
  • Mathias Pribil,

    1. Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, D-82152 Planegg-Martinsried, Germany
    Search for more papers by this author
  • Peter Jahns,

    1. Plant Biochemistry, Heinrich-Heine-University Düsseldorf, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
    Search for more papers by this author
  • Martin Kater,

    1. Dipartimento di Bioscienze, Università degli studi di Milano, I-20133 Milano, Italy
    Search for more papers by this author
  • Dario Leister,

    Corresponding author
    1. Lehrstuhl für Molekularbiologie der Pflanzen (Botanik), Department Biologie I, Ludwig-Maximilians-Universität München, D-82152 Planegg-Martinsried, Germany
    Search for more papers by this author
  • Paolo Pesaresi

    1. Dipartimento di Bioscienze, Università degli studi di Milano, I-20133 Milano, Italy
    Search for more papers by this author

(e-mail leister@lrz.uni-muenchen.de).

Summary

A lack of individual plastid ribosomal proteins (PRPs) can have diverse phenotypic effects in Arabidopsis thaliana, ranging from embryo lethality to compromised vitality, with the latter being associated with photosynthetic lesions and decreases in the expression of plastid proteins. In this study, reverse genetics was employed to study the function of eight PRPs, five of which (PRPS1, -S20, -L27, -L28 and -L35) have not been functionally characterised before. In the case of PRPS17, only leaky alleles or RNA interference lines had been analysed previously. PRPL1 and PRPL4 have been described as essential for embryo development, but their mutant phenotypes are analysed in detail here. We found that PRPS20, -L1, -L4, -L27 and -L35 are required for basal ribosome activity, which becomes crucial at the globular stage and during the transition from the globular to the heart stage of embryogenesis. Thus, lack of any of these PRPs leads to alterations in cell division patterns, and embryo development ceases prior to the heart stage. PRPL28 is essential at the latest stages of embryo–seedling development, during the greening process. PRPS1, -S17 and -L24 appear not to be required for basal ribosome activity and the organism can complete its entire life cycle in their absence. Interestingly, despite the prokaryotic origin of plastids, the significance of individual PRPs for plant development cannot be predicted from the relative phenotypic severity of the corresponding mutants in prokaryotic systems.

Ancillary