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Figure S1. Alternative splicing of SCL33 pre-mRNA in 3-week-old leaves and protoplasts isolated from 3-week-old leaves.

Figure S2. Alignment of sequences of splicevariants produced from the third intron of SCL33 inprotoplasts with splice variants produced from the same intron inthe GFP–INT–GFP splicing reporter, and sequences ofwild-type and mutated 92 nt segments.

Figure S3. SR45 does not bind P4 RNA, and an excess amount of cold P1 RNA does not abolish binding of P4 RNA to SCL33.

Figure S4. Transcripts corresponding to theregion prior to the T-DNA insertion site are not present inthe scl33 mutant.

Figure S5. Alignment of the nucleotide sequenceof intron 3 of SCL33 homologs from Arabidopsisthaliana (At), Capsella rubella (Cr),Brassica rapa (Br) and Populus trichocarpa(Pt).

Figure S6. Nucleotide sequence of SCL33intron 3 of Brachypodium distachyon.

Table S1. List of primers used in genomic PCR, RT-PCR and in preparing all constructs used in this study.

Table S2. Introns with GAAG repeats in the Arabidopsis genome.

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