SEARCH

SEARCH BY CITATION

Figure S1. Effects on 5S rRNA accumulation of combining pnp and rnr mutations.

qRT-PCR was used to measure the amount of sequences containing mature 5S rRNA. Reverse transcription was performed using 3’ qRT-PCR gene-specific primers as described in Sharwood et al. (2011). The results were normalized to actin, and standard error is shown. The amount of 5S rRNA was set to 1 for A263, which acts as a wild-type control. The data are derived from two biological replicates and three technical replicates for each genotype.

Figure S2. RNA phenotypes of single and double mutants. RNA gel blots were probed with the segments shown in the lower right corner, with their extents indicated by underlining on the schematics.

Figure S3. Polysomal loading in WT and rnr1-3. Polysomes were separated in sucrose gradients as shown across the top, and RNA from fractions was analyzed by gel blot.

Figure S4. Analysis of psbC, rbcL and psbA mRNA in ActD-treated WT and rnr1-3 plants. The top part of the figure shows RNA accumulation by gel blot, with numbers at the left corresponding to transcripts whose accumulation is graphed below, as described in the legend to Figure 7.

Figure S5. Analysis of additional chloroplast transcripts following ActD treatment in WT and rnr1-3 plants.

Figure S6. Accumulation of RBCS mRNA in ActD-treated WT and rnr1-3 plants, as described in the legend to Figure 7.

Figure S7. Analysis of mitochondrial cox1, atp9 and rps4 mRNAs in ActD-treated WT and rnr1-3 plants.

Table S1. Sequences of oligonucleotides primers used in this study.

As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.

FilenameFormatSizeDescription
tpj12006_sm_FiguresS1-S7-TableS1.pptx12497KSupporting info item
tpj12006_sm_Supporting-Information-legends.docx17KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.