These authors contributed equally.
Differential remodeling of the lipidome during cold acclimation in natural accessions of Arabidopsis thaliana
Article first published online: 19 OCT 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 6, pages 972–982, December 2012
How to Cite
Degenkolbe, T., Giavalisco, P., Zuther, E., Seiwert, B., Hincha, D. K. and Willmitzer, L. (2012), Differential remodeling of the lipidome during cold acclimation in natural accessions of Arabidopsis thaliana. The Plant Journal, 72: 972–982. doi: 10.1111/tpj.12007
- Issue published online: 6 DEC 2012
- Article first published online: 19 OCT 2012
- Accepted manuscript online: 13 OCT 2012 08:23AM EST
- Received 10 July 2012; revised 21 August 2012; accepted 29 August 2012; published online 19 October 2012.
- freezing tolerance;
- cold acclimation;
- natural variation;
- Arabidopsis thaliana ;
- storage lipids
Freezing injury is a major factor limiting the geographical distribution of plant species and the growth and yield of crop plants. Plants from temperate climates are able to increase their freezing tolerance during exposure to low but non-freezing temperatures in a process termed cold acclimation. Damage to cellular membranes is the major cause of freezing injury in plants, and membrane lipid composition is strongly modified during cold acclimation. Forward and reverse genetic approaches have been used to probe the role of specific lipid-modifying enzymes in the freezing tolerance of plants. In the present paper we describe an alternative ecological genomics approach that relies on the natural genetic variation within a species. Arabidopsis thaliana has a wide geographical range throughout the Northern Hemisphere with significant natural variation in freezing tolerance that was used for a comparative analysis of the lipidomes of 15 Arabidopsis accessions using ultra-performance liquid chromatography coupled to Fourier-transform mass spectrometry, allowing the detection of 180 lipid species. After 14 days of cold acclimation at 4°C the plants from most accessions had accumulated massive amounts of storage lipids, with most of the changes in long-chain unsaturated triacylglycerides, while the total amount of membrane lipids was only slightly changed. Nevertheless, major changes in the relative amounts of different membrane lipids were also evident. The relative abundance of several lipid species was highly correlated with the freezing tolerance of the accessions, allowing the identification of possible marker lipids for plant freezing tolerance.