Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of tail-anchored proteins
Version of Record online: 28 DEC 2012
© 2012 The Authors The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 73, Issue 5, pages 850–861, March 2013
How to Cite
Formighieri, C., Cazzaniga, S., Kuras, R. and Bassi, R. (2013), Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of tail-anchored proteins. The Plant Journal, 73: 850–861. doi: 10.1111/tpj.12077
- Issue online: 22 FEB 2013
- Version of Record online: 28 DEC 2012
- Accepted manuscript online: 20 NOV 2012 08:54AM EST
- Manuscript Accepted: 15 NOV 2012
- Manuscript Revised: 13 NOV 2012
- Manuscript Received: 28 MAR 2012
Figure S1. Sequence alignment of ARSA-homolog proteins. The cDNA of Chlamydomonas reinhardtii ARSA1.
Figure S2. Immunoblot analysis of photosynthetic polypeptides on thylakoid preparations of cw15 and as1 mutant, loaded on a per chlorophyll basis.
Figure S3. Reactivity of α-ARSA1 antiserum against fractions from differential centrifugation of Chlamydomonas cw15 cells.
Figure S4. Reaction of the α-ARSA1 antiserum versus cw15 and as1 total cell extract and versus ARSA1 recombinant protein purified from Escherichia coli by Ni + + column.
|tpj12077-sup-0002-TableS1.docx||Word document||14K||Table S1. Primers used to characterize the arsa1 mutation and for cloning and expression of recombinant ARSA1 in Escherichia coli.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.