Figure S1. Immunostaining of Chinese Spring by TaCENH3-antibody.

Figure S2. Western blotting of wheat CENH3 antibody with rice, maize, barley, common wheat, T. boeoticum, A. speltoides and A. tauschii.

Figure S3. Southern hybridization of wheat and its relatives with LTR retroelements (the same filter).

Figure S4. In situ hybridization of Chinese Spring with centromere-related satellite repeats.

Figure S5. Dot-matrix analysis of centromeric satellite-like repeats in Chinese Spring Contig310431. There are two parallel lines beside the diagonal, indicating that there are repeats in the same orientation.

Figure S6. Three centromeric satellite-like repeats in the same orientation were detected in Contig310431, with lengths 502, 489 and 503 bp, respectively.

Figure S7. Southern hybridization of wheat and its relatives with centromere-related satellite repeats.

tpj12086-sup-0002-TableS1.xlsxapplication/msexcel16KTable S1. Molecular markers and primers designed to screen the 3B physical map with markers derived from genes expected to be located at the 3B centromere.
tpj12086-sup-0003-TableS2.xlsxapplication/msexcel13KTable S2. 3B MTP screening with molecular markers derived from wheat ESTs homologous to rice genes annotated between LOC_Os01 g36860 and LOC_Os01 g37800.
tpj12086-sup-0003-TableS3.docxWord document22KTable S3. PCR primers used in the ChIP analysis.
tpj12086-sup-0007-Legends.docWord document27K 

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