An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana

Authors

  • Hung M. Nguyen,

    1. Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany
    2. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
    Current affiliation:
    1. Center of Research and Development, Duy Tan University, Danang City, Vietnam
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    • These authors contributed equally to this paper.
  • Jos H. M. Schippers,

    Corresponding author
    1. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
    • Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany
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    • These authors contributed equally to this paper.
  • Oscar Gõni-Ramos,

    1. Plant Chemetics Laboratory, Max Planck Institute for Plant Breeding Research, Cologne, Germany
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  • Mathias P. Christoph,

    1. Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany
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  • Hakan Dortay,

    1. Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany
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  • Renier A. L. van der Hoorn,

    1. Plant Chemetics Laboratory, Max Planck Institute for Plant Breeding Research, Cologne, Germany
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  • Bernd Mueller-Roeber

    Corresponding author
    1. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
    • Department of Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany
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For correspondence (e-mail schippers@mpimp-golm.mpg.de or bmr@uni-potsdam.de).

Summary

In both animal and plant kingdoms, body size is a fundamental but still poorly understood attribute of biological systems. Here we report that the Arabidopsis NAC transcription factor ‘Regulator of Proteasomal Gene Expression’ (RPX) controls leaf size by positively modulating proteasome activity. We further show that the cis-element recognized by RPX is evolutionarily conserved between higher plant species. Upon over-expression of RPX, plants exhibit reduced growth, which may be reversed by a low concentration of the pharmacological proteasome inhibitor MG132. These data suggest that the rate of protein turnover during growth is a critical parameter for determining final organ size.

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