tpj12103-sup-0001-FigureS1-S8.docxWord document6212K

Figure S1. Simplified model of sterol and sphingolipid biosynthetic pathways in Arabidopsis thaliana.

Figure S2. Phenotypic analysis of Col-0, abcb19-101, cvp1-3, tsc10a-2, abcb19-101 cvp1-3 and abcb19-101 tsc10a-2 grown on plates.

Figure S3. Time course of FM4-64 endocytosis in epidermal cells of Col-0, cvp1-3 and tsc10a-2.

Figure S4. Differential dependence of ABC and auxin transporters on sterols and sphingolipids.

Figure S5. Sucrose density gradient fractionation shows ABCB19 localization shifts after FEN treatment.

Figure S6. Sucbellular localization analysis of ABCB19-GFP after FB1 and FEN treatment.

Figure S7. ABCB19-GFP forms intracellular accumulations after FEN1 and FB1 treatments in the presence of CHX.

Figure S8. ABCB19 folding/maturation is mediated by the immunophilin-like co-chaperone FKBP42.

tpj12103-sup-0002-TableS1.docxWord document14KTable S1. FAMES and sterols (pmol mg−1 fw) in the lines used in this study.
tpj12103-sup-0003-VideoClipS1.avivideo/avi20249KVideo Clip S1. FRAP of intracellular ABCB19-GFP signal recovers within 2 min of photobleaching.
tpj12103-sup-0004-VideoClipS2.avivideo/avi18978KVideo Clip S2. FRAP of intracellular ABCB19-GFP signal proximal to the PM recovers within 2 min of photobleaching.

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