Original Article

OsCYP2, a chaperone involved in degradation of auxin-responsive proteins, plays crucial roles in rice lateral root initiation

  1. Bo Kang1,†,
  2. Zhongchen Zhang2,†,
  3. Lingling Wang1,
  4. Libin Zheng1,
  5. Weihua Mao3,
  6. Meifei Li1,
  7. Yunrong Wu1,
  8. Ping Wu1,
  9. Xiaorong Mo1,*

Article first published online: 4 MAR 2013

DOI: 10.1111/tpj.12106

Issue

The Plant Journal

The Plant Journal

Volume 74, Issue 1, pages 86–97, April 2013

Additional Information

How to Cite

Kang, B., Zhang, Z., Wang, L., Zheng, L., Mao, W., Li, M., Wu, Y., Wu, P. and Mo, X. (2013), OsCYP2, a chaperone involved in degradation of auxin-responsive proteins, plays crucial roles in rice lateral root initiation. The Plant Journal, 74: 86–97. doi: 10.1111/tpj.12106

Author Information

  1. 1

    State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, China

  2. 2

    College of Agriculture, Northeast Agricultural University, Harbin, China

  3. 3

    Center of Analysis and Measurement, Zhejiang University, Hangzhou, China

  1. †

    These authors contributed equally to this work.

*For correspondence (e-mail xiaorong@zju.edu.cn).

  1. †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 19 MAR 2013
  2. Article first published online: 4 MAR 2013
  3. Accepted manuscript online: 5 JAN 2013 11:35AM EST
  4. Manuscript Accepted: 20 DEC 2012
  5. Manuscript Revised: 18 DEC 2012
  6. Manuscript Received: 3 AUG 2012

Funded by

  • National High Technology Research and Development Program of China 863. Grant Number: 2007AA10Z118
  • National Basic Research and Development Program of China. Grant Number: 2011CB100300
  • Fundamental Research Funds for the Central Universities

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Keywords:

  • cyclophilin;
  • lateral root initiation;
  • auxin;
  • protein interaction;
  • Oryza sativa

Summary

Auxin plays a pivotal role in many facets of plant development. It acts by inducing the interaction between auxin-responsive [auxin (AUX)/indole-3-acetic acid (IAA)] proteins and the ubiquitin protein ligase SCFTIR to promote the degradation of the AUX/IAA proteins. Other cofactors and chaperones that participate in auxin signaling remain to be identified. Here, we characterized rice (Oryza sativa) plants with mutations in a cyclophilin gene (OsCYP2). cyp2 mutants showed defects in auxin responses and exhibited a variety of auxin-related growth defects in the root. In cyp2 mutants, lateral root initiation was blocked after nuclear migration but before the first anticlinal division of the pericycle cell. Yeast two-hybrid and in vitro pull-down results revealed an association between OsCYP2 and the co-chaperone Suppressor of G2 allele of skp1 (OsSGT1). Luciferase complementation imaging assays further supported this interaction. Similar to previous findings in an Arabidopsis thaliana SGT1 mutant (atsgt1b), degradation of AUX/IAA proteins was retarded in cyp2 mutants treated with exogenous 1-naphthylacetic acid. Our results suggest that OsCYP2 participates in auxin signal transduction by interacting with OsSGT1.

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