tpj12129-sup-0002-SupplementalMethods.docxWord document15KMethods S1. Anthocyanin inductive growth conditions and histochemical analysis methods.

Table S1. Naringenin-repressed genes in wild-type and/or tt5.

Table S2. Expression of the candidate genes in wild-type and pap1-D over-expressor.

Table S3. Microarray relative expression values and fold changes of flavonoid and phenylpropamoid genes after naringenin treatment of wild-type and tt5 seedlings.

Table S4. List of phytohormone related-genes from Arabidopsis metabolic pathways.

Table S5. Primer sequences for quantitative RT-PCR analyses.


Figure S1. Validation of genes by quantitative RT-PCR: genes regulated by naringenin.

Figure S2. Validation of genes by quantitative RT-PCR: genes regulated by CHI.

Figure S3. Functional classification of genes showing significantly higher or lower expression levels in non-treated versus naringenin-treated seedlings.

Figure S4. Anthocyanin content in tt6-1, tt3-1 and fls1-1 mutants and wild-types (Ler and WS) seedlings grown in AIC with and without naringenin treatment.

Figure S5. JA biosynthetic pathway.

Figure S6. JA cluster induced by naringenin in tt5.

Figure S7. Expression of JA biosynthetic genes in wild-type and tt5 after naringenin treatment.

Figure S8. Expression of LOX3 and OPR3 in tt5 and wild-type after wounding.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.