A dominant-negative form of Arabidopsis AP-3 β-adaptin improves intracellular pH homeostasis
Version of Record online: 21 MAR 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 74, Issue 4, pages 557–568, May 2013
How to Cite
Niñoles, R., Rubio, L., García-Sánchez, M. J., Fernández, J. A., Bueso, E., Alejandro, S. and Serrano, R. (2013), A dominant-negative form of Arabidopsis AP-3 β-adaptin improves intracellular pH homeostasis. The Plant Journal, 74: 557–568. doi: 10.1111/tpj.12138
- Issue online: 11 MAY 2013
- Version of Record online: 21 MAR 2013
- Accepted manuscript online: 8 FEB 2013 06:59AM EST
- Manuscript Accepted: 3 FEB 2013
- Manuscript Revised: 28 JAN 2013
- Manuscript Received: 8 NOV 2012
- Ministerio de Ciencia e Innovación. Grant Number: BFU2008-00604
- Consellería de Educación. Grant Number: PROMETEO/2010/038
- JAE-preDOC contract
Figure S1. The wat1-1D mutant has a single insertion of T-DNA (a) that segregates with the tolerance to intracellular acidification by acetic acid (b).
Figure S2. The morphological phenotype of wat1-1D in normal conditions is slightly different to that of the wild type.
Figure S3. Phenotypes of wat1-1D mutant are not due to over-expression of At3g55470 gene.
Figure S4. Northern analysis of At3g55480 mRNA from wild type (wt), wat1-1D mutant, and two transgenic lines over-expressing the truncated At3g55480 gene (T3(1) and T3(2)).
Figure S5. The wat1-1D mutant has similar phenotypes than the pat2 mutant.
Figure S6. Initial rate of acetic acid uptake is not affected in the wat1-1D mutant.
Figure S7. Malate consumption during acetic acid treatment is not affected in the wat1-1D mutant.
Figure S8. The efflux of radioactivity after loading with 14C-acetic acid corresponds to acetic acid.
Figure S9. The vacuolar channels TPK1 and ALMT9 are not miss-localized in the wat1-1D mutant.
|tpj12138-sup-0002-SupInfoLegends.docx||Word document||100K||Data S1. Selection of At3g55470 knock-out Arabidopsis lines. Extraction and analysis of nucleic acids. Subcellular localization of K+ channel TPK1 and anion channel AMLT9.|
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