tpj12138-sup-0001-FigS1-S9.docxWord document3836K

Figure S1. The wat1-1D mutant has a single insertion of T-DNA (a) that segregates with the tolerance to intracellular acidification by acetic acid (b).

Figure S2. The morphological phenotype of wat1-1D in normal conditions is slightly different to that of the wild type.

Figure S3. Phenotypes of wat1-1D mutant are not due to over-expression of At3g55470 gene.

Figure S4. Northern analysis of At3g55480 mRNA from wild type (wt), wat1-1D mutant, and two transgenic lines over-expressing the truncated At3g55480 gene (T3(1) and T3(2)).

Figure S5. The wat1-1D mutant has similar phenotypes than the pat2 mutant.

Figure S6. Initial rate of acetic acid uptake is not affected in the wat1-1D mutant.

Figure S7. Malate consumption during acetic acid treatment is not affected in the wat1-1D mutant.

Figure S8. The efflux of radioactivity after loading with 14C-acetic acid corresponds to acetic acid.

Figure S9. The vacuolar channels TPK1 and ALMT9 are not miss-localized in the wat1-1D mutant.

tpj12138-sup-0002-SupInfoLegends.docxWord document100KData S1. Selection of At3g55470 knock-out Arabidopsis lines. Extraction and analysis of nucleic acids. Subcellular localization of K+ channel TPK1 and anion channel AMLT9.
tpj12138-sup-0003-SupplementMethod.docxWord document190K 

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