Phenotypic changes associated with RNA interference silencing of chalcone synthase in apple (Malus × domestica)
Article first published online: 14 MAR 2013
© 2013 The Authors The Plant Journal © 2013 Blackwell Publishing Ltd
The Plant Journal
Volume 74, Issue 3, pages 398–410, May 2013
How to Cite
Dare, A. P., Tomes, S., Jones, M., McGhie, T. K., Stevenson, D. E., Johnson, R. A., Greenwood, D. R. and Hellens, R. P. (2013), Phenotypic changes associated with RNA interference silencing of chalcone synthase in apple (Malus × domestica). The Plant Journal, 74: 398–410. doi: 10.1111/tpj.12140
- Issue published online: 23 APR 2013
- Article first published online: 14 MAR 2013
- Accepted manuscript online: 8 FEB 2013 10:43AM EST
- Manuscript Accepted: 24 JAN 2013
- Manuscript Revised: 21 DEC 2012
- Manuscript Received: 5 JUL 2012
- New Zealand Foundation for Research, Science and Technology. Grant Number: C06X0812
Figure S1. Chalcone synthase (CHS) activity assay showing naringenin formed by transiently expressed CHS protein extracts.
Figure S2. Methodology for transient expression of three chalcone synthase (CHS) proteins and CHS activity assay.
Figure S3. Quantitative RT-PCR analysis of three CHS genes in ‘Royal Gala’ roots, bark, leaves and three developmental stages of fruit skin and cortex.
Figure S4. (a) Primers used for GenomeWalker® analysis of T-DNA integration sites. (b) The sequence data obtained using Genome Walker® amplifications revealed that lines A2, A6 and A7 have different genomic DNA regions alongside the T-DNA right border.
Figure S5. Specificity of CHS-1, CHS-2 and CHS-3 qPCR primers.
Figure S6. Chalcone synthase activity in ‘Royal Gala’ and CHS-silenced lines A3 and A5.
Figure S7. Total phenolic compounds in the axillary buds of four chalcone synthase silenced lines and wild type ‘Royal gala’.
Figure S8. Quantitative RT-PCR analysis of MdPKS-2 expression in four CHS-silenced lines.
Figure S9. An LC-MS analysis of fruit from ‘Royal Gala’ and one CHS-silenced line (Line A5).
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