Arabidopsis thaliana bZIP44: a transcription factor affecting seed germination and expression of the mannanase-encoding gene AtMAN7
Article first published online: 8 APR 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 74, Issue 5, pages 767–780, June 2013
How to Cite
Iglesias-Fernández, R., Barrero-Sicilia, C., Carrillo-Barral, N., Oñate-Sánchez, L. and Carbonero, P. (2013), Arabidopsis thaliana bZIP44: a transcription factor affecting seed germination and expression of the mannanase-encoding gene AtMAN7. The Plant Journal, 74: 767–780. doi: 10.1111/tpj.12162
- Issue published online: 28 MAY 2013
- Article first published online: 8 APR 2013
- Accepted manuscript online: 5 MAR 2013 01:06PM EST
- Manuscript Accepted: 26 FEB 2013
- Manuscript Received: 11 DEC 2012
Figure S1. Phylogenetic tree and schematic distribution of conserved motives among the deduced protein sequences of the endo-β-mannanase proteins of the Brassicaceae species used in this study.
Figure S2. Subcellular localization of the AtMAN7 protein.
Figure S3. Histochemical localization of GUS in 24-h transgenic adult roots of PMAN7-1::GUS, PMAN7-2::GUS, PMAN7-3::GUS.
Figure S4. Heatmap representing expression levels of AtbZIP44 (At1 g75390), AtbZIP11 (At4 g34590) AtbZIP1 (At5 g49450), AtbZIP2 (At2 g18160), AtbZIP4 (At1 g59530) AtbZIP53 (At3 g62420), AtbZIP10 (At4 g02640) and AtbZIP25 (At3 g54620) during seed germination.
Figure S5. Transcription levels of housekeeping gene (18s-RNA), presented as Ct mean values, during seed germination of Arabidopsis thaliana Col-0 seeds and during silique development of A. thaliana Col-0.
Figure S6. Transcription levels of housekeeping gene (18s-RNA), presented as Ct mean values, during seed germination of Arabidopsis thaliana Ws-4, knock-out (KO) bZIP44-1, KO bZIP44-2 and KO MAN7-1 seeds.
Figure S7. Transcription levels of housekeeping gene (18s-RNA), presented as Ct mean values, during seed germination of A. thaliana Col-0, oex-bZIP44-1, and oex-bZIP44-2 seeds.
Table S1. Major characteristics of Brassicaceae predicted endo-β-mannanase proteins.
Table S2. Sequences of conserved amino acids motives (MEME; Bailey et al., 2006) of the endo-β-mannanases from the Brassicaceae species in Figure S1.
Table S3. Promoter sequences of MAN7 genes of the Brassicaceae used in the phylogenetic shadowing analysis.
Table S4. List of primers used for cloning, for probe synthesis in fluorescent in situ hybridization and for genotype T-DNA insertion mutant lines.
Table S5. Characteristics and sequences of the primers used in the RT-qPCR analysis.
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