Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Article first published online: 27 APR 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 74, Issue 6, pages 1029–1044, June 2013
How to Cite
Qian, P., Han, B., Forestier, E., Hu, Z., Gao, N., Lu, W., Schaller, H., Li, J. and Hou, S. (2013), Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis. The Plant Journal, 74: 1029–1044. doi: 10.1111/tpj.12190
- Issue published online: 6 JUN 2013
- Article first published online: 27 APR 2013
- Accepted manuscript online: 30 MAR 2013 06:59AM EST
- Manuscript Accepted: 25 MAR 2013
- Manuscript Revised: 22 MAR 2013
- Manuscript Received: 6 FEB 2013
- National Natural Science Foundation of China. Grant Numbers: 31070247, 91017002, 31070247, 91017002, 31271460
- National Basic Research Program of China. Grant Number: 2009CB941500
- Ministry of Agriculture of the People's Republic of China. Grant Numbers: 2011ZX08009-003-002, 2011ZX08009-003-002, and 2009ZX08009-029B
Figure S1. Complementation of the fk–J3158 mutant phenotype by the wild-type gene.
Figure S2. Seedling phenotypes of fk–J3158, fk–J79 and fk–X224.
Figure S3. Phenotypes of leaf, vascular tissues, root and embryo of fk–J3158.
Figure S4. The sterol C–14 reduction step, and sterols included in the present study.
Figure S5. The post-cycloeucalenol branch of the sterol biosynthetic pathway acts independently of the downstream SMT2/CVP1, DWF5 and BR pathways in stomatal development.
Figure S6. The sterol biosynthetic pathway acts independently of the stomatal development regulators EPF1, EPF2, YDA and BASL.
Figure S7. fk–J3158 stomatal lineage histories in various cases of cell division and differentiation.
Figure S8. Expression patterns of TUB6, TMM and BASL in fk–J3158 and FEN-treated Col-0.
Figure S9. RNA–Seq analysis of cell cycle-related gene expression in 15-day-old fk–J3158 and FEN-treated Col-0.
Table S1. Numbers of differentiated cells identified in fk allele mutants, FEN-treated Col-0, and other mutants of the early steps of the sterol biosynthetic pathway.
Table S2. Numbers of differentiated cells identified in Col-0 and fk–J3158 after treatment with 24–epibrassinolide, stigmasterol or campesterol.
Table S3. Summary of defect phenotypes in mutants of the early steps of the sterol biosynthetic pathway in Arabidopsis.
Table S4. Primers used in this study.
Methods S1. Plant materials.
Methods S2. Imaging and microscopy analysis.
|tpj12190-sup-0002-DataS1.xls||application/msexcel||124K||Data S1. Clustering analysis of the union of differentially expressed genes in fk–J3158 and FEN-treated Col-0.|
|tpj12190-sup-0003-MovieS1.AVI||video/avi||6397K||Movies S1–S3. Time-lapse imaging of TUB6–GFP in dividing small-cell clusters of fk–J3158.|
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