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Figure S1. Complementation of the fk–J3158 mutant phenotype by the wild-type gene.

Figure S2. Seedling phenotypes of fk–J3158, fk–J79 and fk–X224.

Figure S3. Phenotypes of leaf, vascular tissues, root and embryo of fk–J3158.

Figure S4. The sterol C14 reduction step, and sterols included in the present study.

Figure S5. The post-cycloeucalenol branch of the sterol biosynthetic pathway acts independently of the downstream SMT2/CVP1, DWF5 and BR pathways in stomatal development.

Figure S6. The sterol biosynthetic pathway acts independently of the stomatal development regulators EPF1, EPF2, YDA and BASL.

Figure S7. fk–J3158 stomatal lineage histories in various cases of cell division and differentiation.

Figure S8. Expression patterns of TUB6, TMM and BASL in fk–J3158 and FEN-treated Col-0.

Figure S9. RNASeq analysis of cell cycle-related gene expression in 15-day-old fk–J3158 and FEN-treated Col-0.

Table S1. Numbers of differentiated cells identified in fk allele mutants, FEN-treated Col-0, and other mutants of the early steps of the sterol biosynthetic pathway.

Table S2. Numbers of differentiated cells identified in Col-0 and fk–J3158 after treatment with 24epibrassinolide, stigmasterol or campesterol.

Table S3. Summary of defect phenotypes in mutants of the early steps of the sterol biosynthetic pathway in Arabidopsis.

Table S4. Primers used in this study.

Methods S1. Plant materials.

Methods S2. Imaging and microscopy analysis.

tpj12190-sup-0002-DataS1.xlsapplication/msexcel124KData S1. Clustering analysis of the union of differentially expressed genes in fk–J3158 and FEN-treated Col-0.
tpj12190-sup-0003-MovieS1.AVIvideo/avi6397KMovies S1S3. Time-lapse imaging of TUB6GFP in dividing small-cell clusters of fk–J3158.
tpj12190-sup-0004-MovieS2.AVIvideo/avi1952K 
tpj12190-sup-0005-MovieS3.AVIvideo/avi929K 

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