These authors contributed equally to this work.
Genome-wide transcript analysis of early maize leaf development reveals gene cohorts associated with the differentiation of C4 Kranz anatomy
Article first published online: 8 JUN 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 75, Issue 4, pages 656–670, August 2013
How to Cite
Wang, P., Kelly, S., Fouracre, J. P. and Langdale, J. A. (2013), Genome-wide transcript analysis of early maize leaf development reveals gene cohorts associated with the differentiation of C4 Kranz anatomy. The Plant Journal, 75: 656–670. doi: 10.1111/tpj.12229
- Issue published online: 9 AUG 2013
- Article first published online: 8 JUN 2013
- Accepted manuscript online: 7 MAY 2013 04:45AM EST
- Manuscript Accepted: 1 MAY 2013
- Manuscript Revised: 29 APR 2013
- Manuscript Received: 26 MAR 2013
Photosynthesis underpins the viability of most ecosystems, with C4 plants that exhibit ‘Kranz’ anatomy being the most efficient primary producers. Kranz anatomy is characterized by closely spaced veins that are encircled by two morphologically distinct photosynthetic cell types. Although Kranz anatomy evolved multiple times, the underlying genetic mechanisms remain largely elusive, with only the maize scarecrow gene so far implicated in Kranz patterning. To provide a broader insight into the regulation of Kranz differentiation, we performed a genome-wide comparative analysis of developmental trajectories in Kranz (foliar leaf blade) and non-Kranz (husk leaf sheath) leaves of the C4 plant maize. Using profile classification of gene expression in early leaf primordia, we identified cohorts of genes associated with procambium initiation and vascular patterning. In addition, we used supervised classification criteria inferred from anatomical and developmental analyses of five developmental stages to identify candidate regulators of cell-type specification. Our analysis supports the suggestion that Kranz anatomy is patterned, at least in part, by a SCARECROW/SHORTROOT regulatory network, and suggests likely components of that network. Furthermore, the data imply a role for additional pathways in the development of Kranz leaves.