tpj12237-sup-0001-FigsS1-S13.docxWord document15733K

Figure S1. Immunolocalization of UfRTP1p in the extra-haustorial matrix.

Figure S2. Immunolocalization of UsRTP1p in protuberances of the extra-haustorial membrane.

Figure S3. Test for semi-permeability of the extra-haustorial membrane.

Figure S4. Comparison of RTP1p antibodies S746p, S844p and S849p in the pathosystems U. fabae on V. faba and U. striatus on M. sativa.

Figure S5. Immunolocalization using denaturating and non-denaturating conditions on U. fabae-infected V. faba plants.

Figure S6. Multimer formation of purified de-glycosylated RTP1p.

Figure S7. Heterologous expression of GFP–RTP1p and localization of RTP1p in protoplasts.

Figure S8. In silico prediction of aggregation domains in RTP1p.

Figure S9. Capability of filament formation of the RTP1p135-155 peptide and phenylalanine [RIGHTWARDS ARROW] alanine mutants.

Figure S10. Thioflavin T measurement to validate β–aggregation in RTP1p135-155.

Figure S11. Comparative analyses of β–aggregation efficiency using the RTP1p135-155 peptide and phenylalanine [RIGHTWARDS ARROW] alanine mutants.

Figure S12. Thioflavin T stain co-localizes with the RTP1p immuno signal in the extra-haustorial matrix.

Figure S13. Model summarizing structural features at the haustorial interface and localization of RTP1p.

tpj12237-sup-0002-TableS1.docxWord document45KTable S1. Defining the interaction within the U. fabae/V. faba pathosystem at the cellular level.
tpj12237-sup-0003-StagetA.avivideo/avi8453KVideo S1. Early undifferentiated haustorium. Cycloses inside the infected cell is not effected by the first step of compatible interaction.
tpj12237-sup-0004-StagetB.avivideo/avi10220KVideo S2. Haustorium inside a parenchymatic cell that show initial stage of elongation. During this stage, the plant nucleus stays in close contact with the pathogen.
tpj12237-sup-0005-StagetC.avivideo/avi16186KVideo S3. Late stage of infection with a mature haustorium. Not only the nucleus stays in close proximity to the haustorium, but chloroplasts accumulate showing a slow down of cyclosis of these organelles.

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