tpj12240-sup-0001-FigS1.tifimage/tif1140KFigure S1. Gene structure of ARC5 and the mutation in arc5-3.
tpj12240-sup-0002-FigS2.tifimage/tif1022KFigure S2. Map-based cloning of CPD45.
tpj12240-sup-0003-FigS3.tifimage/tif5278KFigure S3. Chloroplast division phenotypes of the mutant alleles of cpd25 and cpd45.
tpj12240-sup-0004-FigS4.tifimage/tif1895KFigure S4. FRS4/CPD25 and FHY3/CPD45 do not regulate the expression of PDV1 and PDV2.
tpj12240-sup-0005-FigS5.tifimage/tif2808KFigure S5. Identification of the promoter region and 5′-untranslated region of ARC5.
tpj12240-sup-0006-FigS6.tifimage/tif2954KFigure S6. Protein sequence alignment of FHY3/CPD45 in Arabidopsis and its closest homolog in various species.
tpj12240-sup-0007-FigS7.tifimage/tif1943KFigure S7. The ARC5 promoter is active in yeast.
tpj12240-sup-0008-FigS8.tifimage/tif797KFigure S8. FHY3/CPD45 has gene activation activity in yeast, whereas FRS4/CPD25 does not.
tpj12240-sup-0009-FigS9.tifimage/tif3645KFigure S9. Negative controls for the BiFC analysis presented in Figure 10a.
tpj12240-sup-0010-TableS1-S3.docWord document60K

Table S1. Markers and primers used for the fine mapping of CPD25.

Table S2. Markers and primers used for the fine mapping of CPD45.

Table S3. Primers used for the identification of the 5′ -UTR and promoter region of ARC5 as shown in Figure S5.

tpj12240-sup-0011-MethodS1-S7.docWord document58K

Methods S1. Constructs for plant transformation.

Methods S2. RT-PCR and qRT-PCR analysis.

Methods S3. Recombinant protein expression and purification.

Methods S4. Electrophoretic mobility shift assay (EMSA).

Methods S5. Particle gun bombardment and GUS staining.

Methods S6. Yeast analysis.

Methods S7. BiFC Assay.

tpj12240-sup-0012-Legends.docWord document35K 

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