Figure S1. Isolation and characterization of the cotton GhCKI gene.

Figure S2. Conserved N- and variable C–termini of casein kinase I (CKI) proteins.

Figure S3. GhCKI expression according to GUS assays and RNA in situ hybridization.

Figure S4. Over-expression of GhCKI blocked the switch from non-embryogenic to embryogenic callus.

Figure S5. Copy number and expression analysis of transgenic Arabidopsis expressing P35S:GhCKI.

Figure S6. Analysis of the phenotypes of transgenic Arabidopsis expressing P35S:GhCKI.

Figure S7. Reciprocal crosses revealed that over-expressing GhCKI caused male sterility.

Figure S8. Expression analyses of AtCKL genes in wild-type, OE25–6 and OE15–7 anthers.

Figure S9. GhCKI over-expression inhibits endothecium wall thickening and causes anther indehiscence.

Figure S10. Transmission electron microscopy analyses of anthers in wild-type, OE25–6 and OE15–7 lines.

Figure S11. Expression of tapetum genes in wild-type, OE25–6 and OE15–7 buds with stage 1–10 anthers.

Figure S12. Expression analyses of ABA and H2O2 homeostasis genes in wild-type, OE25–6 and OE15–7 buds.

Table S1. Genes exhibit contrasting expression patterns in 84021 and H05 under two temperature conditions.

Table S2. Genes with significant changes in expression levels, ranked according to their OE15–7/OE25–6 ratio.

Methods S1. Gene expression profiling, bioinformatic analysis, gene cloning and promoter isolation, construct generation, and plant transformation.


Table S3. Oligonucleotides used in this study.

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