Present address: The author responsible for distribution of materials integral to the findings presented in this article is Guang-Yuh Jauh.
Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Article first published online: 20 JUL 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
The Plant Journal
Volume 75, Issue 6, pages 927–940, September 2013
How to Cite
Bolaños-Villegas, P., Yang, X., Wang, H.-J., Juan, C.-T., Chuang, M.-H., Makaroff, C. A. and Jauh, G.-Y. (2013), Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis. The Plant Journal, 75: 927–940. doi: 10.1111/tpj.12261
- Issue published online: 6 SEP 2013
- Article first published online: 20 JUL 2013
- Accepted manuscript online: 10 JUN 2013 03:24AM EST
- Manuscript Accepted: 29 MAY 2013
- Manuscript Revised: 25 APR 2013
- Manuscript Received: 1 MAR 2013
- Academia Sinica
- National Science and Technology Program for Agricultural Biotechnology. Grant Number: 098S0030055-AA
- National Science Council. Grant Numbers: 99-2321-B-001-036-MY3, MCB0718191
- National Science Foundation
- CTF7/ECO1 ;
- Arabidopsis thaliana ;
- chromatid cohesion;
- DNA repair
The proper transmission of DNA in dividing cells is crucial for the survival of eukaryotic organisms. During cell division, faithful segregation of replicated chromosomes requires their tight attachment, known as sister chromatid cohesion, until anaphase. Sister chromatid cohesion is established during S-phase in a process requiring an acetyltransferase that in yeast is known as Establishment of cohesion 1 (Eco1). Inactivation of Eco1 typically disrupts chromosome segregation and homologous recombination-dependent DNA repair in dividing cells, ultimately resulting in lethality. We report here the isolation and detailed characterization of two homozygous T-DNA insertion mutants for the Arabidopsis thaliana Eco1 homolog, CHROMOSOME TRANSMISSION FIDELITY 7/ESTABLISHMENT OF COHESION 1 (CTF7/ECO1), called ctf7-1 and ctf7-2. Mutants exhibited dwarfism, poor anther development and sterility. Analysis of somatic tissues by flow cytometry, scanning electron microscopy and quantitative real-time PCR identified defects in DNA repair and cell division, including an increase in the area of leaf epidermal cells, an increase in DNA content and the upregulation of genes involved in DNA repair including BRCA1 and PARP2. No significant change was observed in the expression of genes that influence entry into the endocycle. Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced. Transcript levels for several meiotic genes, including the recombinase genes DMC1 and RAD51C and the S-phase licensing factor CDC45 were elevated in mutant anthers. Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.