Figure S1. Identification of BEE, BIM and HFR1 proteins as POF proteins

Figure S2. Cartoon showing the various PAR1 regions and their known functions.

Figure S3. EMSA of GST-fused BEE2 and BIM1.

Figure S4. Effect of increased levels of PAR1 or PAR2 activity and time of simulated shade exposure on specific gene expression.

Figure S5. Hypocotyl elongation of mutant seedlings deficient in several BEE and BIM genes in response to simulated shade.

Figure S6. Spatial patterns of expression of PAR1, BEE1, BEE2, BEE3, BIM1 and BIM2 using the Anatomy tool of Genevestigator.


Methods S1. Analysis of hypocotyl length of bee123 bim13 bim2/+ seedlings.

Methods S2. Primers used for quantitative PCR analyses.

Methods S3. Y2H screening and directed assays.

Methods S4. Generation of constructs.

Methods S5. In vitro pull-down analyses.

Methods S6. Electrophoretic mobility shift assays (EMSAs).

Methods S7. Anatomy tool of Genevestigator.


Table S1. anova table for experiments shown in Figures 1f and 3f and Figure S1K.

Table S2. anova table for experiments shown in Figures 5 and 6.

Table S3. anova table for experiments shown in Figure 8(c,d) and Figure S5.

Table S4. anova table for experiments shown in Figure 9.

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