Figure S1. Isolation of ozs mutants.

Figure S2. Dose-dependent Zn2+ hypersensitivity of ozs1.

Figure S3. The ozs1 phenotype is caused by a mutation in AtMTP1.

Figure S4. Hypersensitivity of ozs2 towards Zn2+ is highly specific.

Figure S5. Zn accumulation of wild-type Col-0 and ozs2 mutant seedlings.

Figure S6. Semi-dominance of the ozs2 Zn2+-hypersensitivity phenotype.

Figure S7. Characterization of the atpme3 T-DNA insertion line 002A10 (GABI-Kat).

Figure S8. AtPME3-overexpressing lines are specifically Zn2+ hypersensitive.

Figure S9. Total pectin methylesterase activity in AtPME3 overexpressers and T-DNA insertion lines.

Figure S10. Ca2+ suppresses the Zn2+-hypersensitivity phenotype caused by overexpression of AtPME3.

Figure S11. Pectin staining of Col-0 and ozs2 roots with monoclonal antibodies JIM7 and CCRC M38.

Figure S12. Root ultrastructure of ozs2 displays no difference from Col-0 wild type.

tpj12279-sup-0002-TableS1-S2.docxWord document17K

Table S1. Cell wall thickness of the different Col-0 and ozs2 root cell types.

Table S2. Primers used.

tpj12279-sup-0003-SupplementLegends.docxWord document21K 

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.