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Figure S1. Root growth pattern of Al-sensitive and Al-resistant Sorghum plants.

Figure S2. High-magnification three-dimensional images of Al-induced reactive oxygen species at the distal part of the transition zone.

Figure S3. Sequence of events involving Al and reactive oxygen species at the cellular level.

Figure S4. Colocalization of Al-induced callose and Al accumulation in root cells.

tpj12290-sup-0002-Methods.docxWord document24KMethods S1. Extended material and methods.
tpj12290-sup-0003-MovieS1.avivideo/avi4094KMovie S1. Confocal laser scanning microscope images showing all optical sections of the Al-resistant live sorghum plant roots revealing reactive oxygen species (green) and propidium iodide (red) localization pattern 1 day after Al treatments in the distal transition zone.
tpj12290-sup-0004-MovieS2.avivideo/avi3142KMovie S2. Confocal laser scanning microscope images showing all optical sections of the Al-sensitive live sorghum plant roots revealing reactive oxygen species (green) and propidium iodide (red) localization pattern 1 day after Al treatments in the transition zone.
tpj12290-sup-0005-MovieS3.avivideo/avi2506KMovie S3. Confocal laser scanning microscope images showing all optical sections of the Al-tolerant live sorghum plant roots revealing reactive oxygen species (green) and propidium iodide (red) localization pattern 1 day after Al treatments in the distal transition zone.
tpj12290-sup-0006-MovieS4.avivideo/avi2817KMovie S4. Confocal laser scanning microscope images showing all optical sections of the Al-sensitive live sorghum plant roots revealing reactive oxygen species (green) and propidium iodide (red) localization pattern 1 day after Al treatments in the DTZ. The root tip is at the right side of the frame. Depth is indicated in the top left corner.
tpj12290-sup-0007-MovieS5.avivideo/avi2271KMovie S5. Confocal laser scanning microscope images showing all optical sections of the Al-tolerant live sorghum plant root revealing reactive oxygen species (green) and propidium iodide (red) localization pattern 3 days after Al treatments.
tpj12290-sup-0008-MovieS6.avivideo/avi3833KMovie S6. Confocal laser scanning microscope images showing all optical sections of the Al-sensitive live sorghum plant root showing reactive oxygen species (green) and propidium iodide (red) localization pattern 3 days after Al treatments in the transition zone.

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