Visualization of plant cell wall lignification using fluorescence-tagged monolignols
Article first published online: 23 AUG 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
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The Plant Journal
Volume 76, Issue 3, pages 357–366, November 2013
How to Cite
Tobimatsu, Y., Wagner, A., Donaldson, L., Mitra, P., Niculaes, C., Dima, O., Kim, J. I., Anderson, N., Loque, D., Boerjan, W., Chapple, C. and Ralph, J. (2013), Visualization of plant cell wall lignification using fluorescence-tagged monolignols. The Plant Journal, 76: 357–366. doi: 10.1111/tpj.12299
- Issue published online: 16 OCT 2013
- Article first published online: 23 AUG 2013
- Accepted manuscript online: 29 JUL 2013 12:32AM EST
- Manuscript Accepted: 18 JUL 2013
- Manuscript Revised: 19 JUN 2013
- Manuscript Received: 29 APR 2013
- US Department of Energy, the Office of Science. Grant Number: DE-SC0006930
- University of Wisconsin Vilas Associate Award
- Stanford University Global Climate and Energy Project (GCEP)
- Scion CORE funding
- Great Lakes Bioenergy Research Center (GLBRC)
- Joint BioEnergy Institute (JBEI)
- Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)
- Energy Frontier Research Center
- US DOE's Office of Science. Grant Numbers: DE-FC02-07ER64494, DE-AC02-05CH11231, DE-SC0000997
Figure S1. Transverse sections (60 μm) of a Pinus radiata stem stained with tetramethylbenzidine (TMB), exhibiting the sites where phenol oxidase activities were detected before (left) and after (right) heat treatment (85°C for 1 h).
Figure S2. A close comparison of labeled and unlabeled sections of Pinus radiata stem at identical exposure showing the contribution of autofluorescence.
Figure S3. Arabidopsis thaliana protoplast cells treated with (left) and without (right) NBD-tagged monolignol (3G, 50 μm for 4 h).
Data S1. NMR spectra of synthetic compounds.
Appendix S1. Synthetic procedures.
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