Accession numbers: NtPPME1(AY772945), AtVSR2 (NM_128582).
Apical F-actin-regulated exocytic targeting of NtPPME1 is essential for construction and rigidity of the pollen tube cell wall
Article first published online: 28 AUG 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
The Plant Journal
Volume 76, Issue 3, pages 367–379, November 2013
How to Cite
Wang, H., Zhuang, X., Cai, Y., Cheung, A. Y. and Jiang, L. (2013), Apical F-actin-regulated exocytic targeting of NtPPME1 is essential for construction and rigidity of the pollen tube cell wall. The Plant Journal, 76: 367–379. doi: 10.1111/tpj.12300
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- Issue published online: 16 OCT 2013
- Article first published online: 28 AUG 2013
- Accepted manuscript online: 30 JUL 2013 02:18AM EST
- Manuscript Accepted: 18 JUL 2013
- Manuscript Revised: 15 JUL 2013
- Manuscript Received: 17 DEC 2012
- Research Grants Council of Hong Kong. Grant Numbers: CUHK466309, 466610, 466011, 465112, CUHK2/CRF/11G, HKUST10/CRF/12R, HKBU1/CRF/10
- NSFC/RGC. Grant Number: N_CUHK406/12
- NSFC. Grant Number: 31270226
- Shenzhen Peacock Project. Grant Number: KQTD201101
- CUHK Schemes
- US National Foundation of Science. Grant Number: MCB0618339
- apical F-actin;
- cell wall;
In tip-confined growing pollen tubes, delivery of newly synthesized cell wall materials to the rapidly expanding apical surface requires spatial organization and temporal regulation of the apical F-actin filament and exocytosis. In this study, we demonstrate that apical F-actin is essential for the rigidity and construction of the pollen tube cell wall by regulating exocytosis of Nicotiana tabacum pectin methylesterase (NtPPME1). Wortmannin disrupts the spatial organization of apical F-actin in the pollen tube tip and inhibits polar targeting of NtPPME1, which subsequently alters the rigidity and pectic composition of the pollen tube cell wall, finally causing growth arrest of the pollen tube. In addition to mechanistically linking cell wall construction and apical F-actin, wortmannin can be used as a useful tool for studying endomembrane trafficking and cytoskeletal organization in pollen tubes.