A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis
Article first published online: 3 OCT 2013
© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd
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The Plant Journal
Volume 76, Issue 3, pages 519–529, November 2013
How to Cite
McHale, M., Eamens, A. L., Finnegan, E. J. and Waterhouse, P. M. (2013), A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis. The Plant Journal, 76: 519–529. doi: 10.1111/tpj.12306
- Issue published online: 16 OCT 2013
- Article first published online: 3 OCT 2013
- Accepted manuscript online: 12 AUG 2013 07:55AM EST
- Manuscript Accepted: 5 AUG 2013
- Manuscript Revised: 26 JUL 2013
- Manuscript Received: 9 JUN 2013
Table S1. Oligonucleotide primer and probe sequences used in this study.
Table S2. sRNA accumulation in the pap1-D background expressing the SSU_hpCHS.
Table S3. amiRNA accumulation in the pap1-D background expressing SSU_amiR21-CHS and SSU_amiR22-CHS.
Table S4. Distribution of sRNA accumulation in the pap1-D background expressing SSU_amiR21-CHS and SSU_amiR22-CHS.
Table S5. Phasing analysis of SSU_amiR22-CHS directed phasiRNA production.
|tpj12306-sup-0002-FigS1.eps||image/eps||89K||Figure S1. A single nucleotide (U) was deleted (−) from the oligonucleotide corresponding to the amiRNA* strand (blue) resulting in a predicted asymmetric ‘bubble’ in the duplex structure.|
|tpj12306-sup-0003-FigS2.eps||image/eps||72K||Figure S2. Diagram of the constructs used in these experiments.|
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