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tpj12354-sup-0001-Legends.docxWord document26K 
tpj12354-sup-0002-FigS1-S7.docxWord document2314K

Figure S1. Schematic diagrams of the Gaussia luciferase (gluc) reporter constructs used for Chlamydomonas transformation.

Figure S2. Expression of the Gaussia luciferase (gluc) reporter transgenes in Chlamydomonas, with or without cre-miR1174.2 target sites in their 3′ UTRs.

Figure S3. Schematic diagrams of the Gaussia luciferase (gluc)/tagged aph7 reporter constructs used for Chlamydomonas transformation.

Figure S4. Monitoring of the potency of cre-miR1174.2-mediated silencing in pools of transgenic Chlamydomonas strains.

Figure S5. The Gaussia luciferase (gluc) reporter gene was silenced in Chlamydomonas to varying degrees when its transcript contained a single target site for miR912, miR910 or a putative miRNA (candidate 82; Zhao et al., 2007).

Figure S6. Nucleotide substitutions in the cre-miR1174.2 target site for the mismatch tolerance test.

Figure S7. A Gaussia luciferase (gluc) reporter containing a target site matching only the miR910 seed region was moderately repressed in Chlamydomonas.

tpj12354-sup-0003-TableS1.docxWord document49KTable S1. Frequency of hygromycin B-resistant transgenic strains in which the whole transforming DNA fragment is integrated.

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